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KMID : 1177720100040010031
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Journal of Alternatives to Animal Experiments
2010 Volume.4 No. 1 p.31 ~ p.44
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Establishment of Screening and Test Methods of Atopic Dermatitis Using In Vitro Systems
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Ju Jung-Hun
Lim Chae-Hyung
Kim Jin-Ho
Lee Jung-Pyo
Sohn Kyung-Hee
Hong Soon-Keun
Kwon Tae-Rin
Chang Mi
Kim Dong-Sup
Yoon Hae-Seong
Park Kui-Lea
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Abstract
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Atopic dermatitis (AD) is a common pruritic disease and its incidence is increasing in infancy and childhood. AD is characterized by extreme pruritus and chronically relapsing inflammations and prevalence of AD has increased in recent years, but its exact etiology is unclear. Antigen?induced release of inflammatory mediators from mast cells causes the immediate symptoms of IgE?mediated allergic diseases, including allergic rhinitis, atopic dermatitis and atopic eczema. Mast cells rapidly release various allergic mediators, including histamine, cytokines that mediated acute and chronic allergic reaction. Since mast cells play a central role in the pathogenesis of various allergic diseases, we investigated the effect of exposure to 18 chemicals on the IgE?mediated allergic response in mast cells. We use two type of mast cells
(RBL?2H3 cells, BMMC) to investigate the effects of chemicals on degranulation and cytokine production.
Geraniol and formaldehyde significantly induced IgE?mediated mast cell degranulation. Dermatophagoides pteronyssinus allergen 1(DP) and dermatophagoides farinae allergen 1(DF) also induced degranulation without statistically significance. Also, we showed that release of IL?1β, IL?6 and GM?CSF was increased by treatment of geraniol. Also, We found that DP and DF induced release of IL?1β, IL?6, GM?CSF and TNF?α. These results suggest that in vitro method using mast cells degranulation and allergic cytokine secretion assay are useful for screening of atopic dermatitis trigger.
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KEYWORD
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Atopic dermatitis, Mast cell, In Vitro
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